THE TECHNOLOGY OF ALiCE

Cell-free synthetic biology is the next breakthrough for genetic and protein research. Free from the limitations of living host systems, cell-free protein expression enables you to achieve rapid results, gain exquisite control over reaction conditions, and reduce background interference. LenioBio’s novel technology ALiCE™ provides the highest cell-free protein expression yields available in a single step.

The ALiCE™ technology is the result of extensive development and validation by industry experts. For years, this valuable tool was exclusively used by biotech industry leaders for their own in-house protein development and production. Now, for the first time, we are pleased to make it available to the wider protein expression community.

Just add DNA template and go!

With LenioBio’s simple, one-step, cell-free protein expression technology ALiCE™, it takes just hours to obtain various “difficult to express” proteins, including:

  • Antigenic vaccine components
  • Antibodies and antibody fragments
  • Peptide and protein hormones
  • Membrane proteins and glycoproteins
  • Cytotoxic proteins

How do we do it?

Cell-free protein expression (CFPE) systems derived from crude cell extracts have been used for decades as a research tool, and have introduced many attractive advantages to protein expression technology. However, a critical barrier to the adoption of CFPE systems as alternatives to cell-based approaches has been the problem of relatively low protein yields.

To address this problem, LenioBio has taken advantage of plant cell biology and is bringing to the market a novel eukaryotic cell-free expression system ALiCE™ that yields significant quantities of protein. We start our proprietary production process with a robust and fast-growing tobacco cell line. We then remove the plant cell vacuoles, which contain harmful hydrolytic enzymes that can degrade lysate performance. What remains is an “almost living” extract containing the complete protein production machinery, including functional mitochondria – the cell’s energy supplying organelles.

ALiCE™ cell-free eukaryotic protein expression kits provide outstanding value for money, lowering your cost to produce one mg of protein by as much as 100-fold, compared to competing kits.

ALiCE™ outperforms all commercial cell-free expression kits in a single step reaction

Our new cell-free protein expression technology ALiCE™ is the result of extensive development and validation by industry experts. For years, this valuable tool remained exclusively in the hands of industry leaders for their own in-house protein production. Now, for the first time, we are pleased to make it available to the wider protein expression community.