Almost Living Cell-Free Expression
ALiCE® is a scalable eukaryotic cell-free protein expression system capable of producing even the most complex proteins in under 48 hours. Our proprietary cell-free lysate contains all of the machinery necessary to implement eukaryotic post-translational modifications, without specific optimizations.
Furthermore, the ALiCE® system consistently produces protein yields of up to an industry-leading 3 grams per liter.
Without the constraints imposed by living systems, our cell-free system has the advantage of being able to produce all proteins, even toxic ones.
> 2 grams per liter protein yield
Complex post-translational modifications
Protein in under 48 hours
Scalable reactions to 10 L
"You have to consider all the things you're going to be supplementing into the reaction. The beauty of the ALiCE system is that all of those components are built into the lysate."
A eukaryotic cell-free platform for reliable expression of functional membrane proteins
Due to their cell surface accessibility and importance in cellular regulation, membrane proteins are significant drug targets. However, they are challenging to obtain due to their aggregation-prone nature, resulting in low expression and purification yields.
ALiCE in action:
- Two human G-coupled receptors (GPCRs) - CB2 and ADRB2 - were produced using the ALiCE cell-free expression system in a 24 hour reaction.
- It was possible to assess GPCR functionality within the native microsome environment without purification.
- The activity profile of CB2 produced using ALiCE was comparable to the protein expressed using a cell-based system.
Conclusion
ALiCE is particularly suitable for membrane protein expression because it contains native microsomes from the endoplasmic reticulum and Golgi. These microsomes act as a scaffold for the insertion of membrane proteins. Unlike cell-based systems, ALiCE does not require cell lysis for downstream processing.
Expression of a functional therapeutic antibody in the ALiCE cell-free protein expression system
Monoclonal antibodies (mAbs) and their related therapeutic antibody variants are essential components in the field of medical treatment, particularly in the management of cancer and autoimmune diseases. These antibodies are engineered to specifically target and interact with particular molecules or cells in the body, thereby offering a highly targeted and precise approach to therapy.
ALiCE in action:
- Adalimumab (aka Humira®), a complex therapeutic antibody with 12 intra- and 4 intermolecular disulfide bonds and 2 glycosylation sites, was successfully produced using the ALiCE system.
- Heavy and light chains were simultaneously expressed in a one-pot reaction, resulting in fully assembled tetrametic antibody in 24 hours.
- Adalimumab produced in ALiCE showed similar antigen binding to CHO-produced antibody.
Conclusion:
The ALiCE cell-free expression system can significantly expedite the process of antibody screening.
Expressing Virus-Like Particles using the Cell-Free Protein Expression System
Virus-Like Particles (VLPs) are multi-subunit proteins that are considered a promising approach for vaccines because they can elicit robust and long-lasting immune responses resembling those generated by viruses. Importantly, they pose fewer safety concerns as they lack the genetic material and machinery necessary for viral replication.
ALiCE in action:
- Using the ALiCE expression system, high quality, fully assembled Hepatitis B Core (HBc) Carrier VLPs were produced.
- VLPs produced by ALiCE are capable of inducing the expected immune response.
Conclusion:
The use of ALiCE is established as a valuable tool for the rapid screening and production of VLP-based vaccine candidates.
Interested in learning more about the benefits of cell-free compared to cell-based expression systems?
How does ALiCE® work?
Producing eukaryotic proteins with yields of up to 3 g/liter couldn't be easier with ALiCE®: simply add plasmid DNA containing your cloned gene-of-interest, incubate for 24-48 hours and retrieve your protein.
Download our White Paper and discover the potential of ALiCE to redefine protein production
ALiCE® as a product
Our ALiCE® lysate is currently available as both a protein expression kit for small-scale expression experiments and as a protein service. If you are interested in purchasing ALiCE® in larger volumes for protein manufacture at scale, please contact our business development team.
ALiCE® as a kit
ALiCE is available as a cell-free protein expression kit in three different sizes to suit your needs.
ALiCE® as a service
We offer an end-to-end protein service from cloning to expression and purification.
ALiCE® at scale
Interested in producing your protein of interest in large amounts? Contact our Sales team​.
Do you have a question about ALiCE?
Contact us and we’ll get right back to you.
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