TECHNOLOGY
WHO IS ALiCE®
Meet ALiCE®, our Almost Living Cell-Free Expression platform. ALiCE® is a universal protein production platform: with it you can make any type of protein within 24 hours.
1
• Full length IgG: Adalimumab, Rituximab• Bi-specific Ab:Faricimab, Emicizumab• scFvs, Fabs, BiTEs• Nanobodies/VHHs: Caplacizumab,
2
• Cytokines: IL-2, IL7• Growth Factors: hFGF2, EGF• Hormones: EPO, Insulin• Fc-fusion: TNFR-Fc• Glycoenzymes: B-glucocerebrosidase• Fluorescent reporters: eYFP, GFP, RFP• Luminescent: Luciferase
3
• GPCRs: CB2, LGR5, ADRB2• RTKs: FGFR3, EGFR• Membrane Pores: Aquaporin• Membrane receptors: CD20
4
• Capsid proteins: Hepatitis B core antigen VLP• Viral antigens: Influenza HA, SARS-CoV 2 RBD, Dengue antigens• Glycoproteins: Hepatitis B surface antigen• Parasite antigens: Malaria, Necator americanus, Schistosoma mansoni, Trypanosoma cruzi
5
• DNA/RNA Enzymes: DNAa, Benzonase• ID/aggregation prone: Tau, alpha-synuclein• Disulfide-rich: GP60, Alteplase• de novo Al-designed
ALiCE® CAN MAKE THE MOST COMPLEX AND UNNATURAL PROTEINS
- ALiCE® derives from a Eukaryotic BY-2 cell background and contains ER-derived microsomes for complex post-translational modifications such as disulfide bonding and N-glycosylation.
- There are no boundaries in ALiCE®, so if your protein requires special cofactors, non-canonical amino acids, or other unusual chaperones, these are simply added to the reaction mix.
ALiCE® IS SCALABLE
You can run your reactions at microlitre to 10s of litre scales with the same results
- The ALiCE® production process has been optimized and scaled over decades of research, first at Dow Agrosciences, and since 2016 at LenioBio GmbH.
- Active mitochondria are present in the lysate for direct energy generation. No ATP or other expensive additions are required!
ALiCE® SOLVES THE PROBLEM
of how to produce proteins for research, and enables scientists to focus on their science, not production.
WHAT MAKES ALiCE® UNIQUE?
ALiCE® is the only platform with translationally active microsomes for complex post-translational modifications and trafficking. This means that:
- Disulfide bridges can be formed
- N-Glycosylation is present
- Membrane proteins can be actively transported into a membrane in a co-translational translocation process which produces proteins that are identical to their natural counterparts
PROVE IT
Here are some case studies on the use of ALiCE® for:
- Antibodies and VHH domains
- Fc Fusion proteins
- Multispecific antibodies
- Virus Like Particles
- Cringle Domain & complex enzymes
- Full length membrane proteins
- Non-canonical amino acid incorporation
HOW DO I TRY THIS?
You can access ALiCE® as a service for one off projects that you need to outsource, or have our team visit your labs and set up the platform for you.