A Powerful Expression System in the Hands of Experts
Our protein service employs a powerful eukaryotic expression system called ALiCE®. Harnessing the power of cell-free expression, ALiCE® has a strong track record producing varied protein classes, including those with complex features such as disordered regions, transmembrane domains and kringle structures.
Our team of protein scientists excels at maximizing the potential of our expression system from cloning strategy to scalable expression and purification. With ALiCE® as our cornerstone, we boast an exceptional expression success rate of nearly 100%, along with a proven history of overcoming expression challenges encountered in other systems.
Our Team Excel at Producing Challenging Proteins
We have produced varied proteins for our customers, many of them highly complex.
These are just a few examples:
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Targets
Membrane proteins and antigens, including a vaccine target with exceptionally low yields in other systems.
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Complex enzymes
A therapeutic enzyme containing kringle domains and multiple glycosylation sites and disulfide bonds.
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Antibody variants
A varied panel of IgGs, scVs and Fabs expressed in a 96-well plate high throughput format.
A Flexible Structure to Fit Your Needs
Discover our suite of ALiCE® Pro-X packages—tailored solutions that evolve with your challenging protein expression needs. Whether you’re exploring expressibility with a comprehensive data package, advancing with optimized yield guarantees, or scaling up production to obtain high-quality, research-ready proteins, our flexible options are designed to drive your project forward.
ALiCE® Pro-X Feasibility
Explore the potential of your challenging proteins and receive a comprehensive data package on expressibility
ALiCE® Pro-X Feasibility
ALiCE® Pro-X Development
Precision-engineered expression to ensure a guaranteed 0.25 mg yield of your most challenging proteins—optimized from DNA design to purified delivery
ALiCE® Pro-X Development
ALiCE® Pro-X Production
Unlock the potential of your challenging proteins with a tailor-made production package that delivers high-quality, research-ready protein at the scale you require
ALiCE® Pro-X ProductionCost-Efficient Scaling
ALiCE® eliminates the need for cell-line optimization for scaled production. Whether expressing simple or complex proteins requiring post-translational modifications or membrane integration, ALiCE® delivers consistent yields from 100 µl to 100 mL and beyond without requiring timely process optimization (Fig. 1 A and B).
With extensive front-loading during the proof of concept and small-scale protein production phases of a project, as you progress to larger-scale protein production with us, you can begin to realize the benefits of economies of scale. With minimal optimization needed for scaling ALiCE® reactions, costs remain manageable as your protein production grows.
Ready to eliminate expression bottlenecks? Let’s discuss how ALiCE® can accelerate your next breakthrough.
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References
Das Gupta et. al., . 2023, J. Biotech. Bioeng.: https://doi.org/10.1002/bit.28461