A Powerful Expression System in the Hands of Experts
Our protein service employs a powerful eukaryotic expression system called ALiCE®. Harnessing the power of cell-free expression, ALiCE® has a strong track record producing varied protein classes, including those with complex features such as disordered regions, transmembrane domains and kringle structures.
Our team of protein scientists excels at maximizing the potential of our expression system from cloning strategy to scalable expression and purification. With ALiCE® as our cornerstone, we boast an exceptional expression success rate of nearly 100%, along with a proven history of overcoming expression challenges encountered in other systems.
Our Team Excel at Producing Challenging Proteins
We have produced varied proteins for our customers, many of them highly complex.
These are just a few examples:
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Targets
Membrane proteins and antigens, including a vaccine target with exceptionally low yields in other systems.
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Complex enzymes
A therapeutic enzyme containing kringle domains and multiple glycosylation sites and disulfide bonds.
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Antibody variants
A varied panel of IgGs, scVs and Fabs expressed in a 96-well plate high throughput format.
A Flexible Structure to Fit Your Needs
Discover our suite of ALiCE® Pro-X packages—tailored solutions that evolve with your challenging protein expression needs. Whether you’re exploring expressibility with a comprehensive data package, advancing with optimized yield guarantees, or scaling up production to obtain high-quality, research-ready proteins, our flexible options are designed to drive your project forward.
ALiCE® Pro-X Screening
Delivering screening of multiple proteins on a 96-well plate
ALiCE® Pro-X Feasibility
Receive approx. 50 µg of your difficult-to-express protein
ALiCE® Pro-X Development
Receive a guaranteed 0.25 mg yield of your most challenging proteins
ALiCE® Pro-X Production
Jumpstart your desired difficult-to-express protein with > 85% purity
Cost-Efficient Scaling
ALiCE® eliminates the need for cell-line optimization for scaled production. Whether expressing simple or complex proteins requiring post-translational modifications or membrane integration, ALiCE® delivers consistent yields from 100 µl to 100 mL and beyond without requiring timely process optimization (Fig. 1 A and B).
With extensive front-loading during the proof of concept and small-scale protein production phases of a project, as you progress to larger-scale protein production with us, you can begin to realize the benefits of economies of scale. With minimal optimization needed for scaling ALiCE® reactions, costs remain manageable as your protein production grows.
Robin Hurst, Senior Research R&D Scientist, Promega Corporation
“LenioBio offers an excellent cell-free protein synthesis system (ALiCE®) and a team of highly knowledgeable, supportive scientists make LenioBio truly a great company to collaborate with. Our work together has included the expression of numerous challenging cytosolic proteins, nanobodies, and antibodies, and so far, we have yet to encounter a protein that ALiCE® couldn’t express effectively.”
Ready to eliminate expression bottlenecks? Let’s discuss how ALiCE® can accelerate your next breakthrough.
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References
Das Gupta et. al., . 2023, J. Biotech. Bioeng.: https://doi.org/10.1002/bit.28461