Video: How to Produce Proteins in 24 Hours with ALiCE®
Our ALiCE® cell-free protein expression kits are designed with ease-of-use in mind.
Using our simple protocol will take you from reaction set-up to expressed protein in 24 hours. All it takes is your gene-of-interest cloned into one of our vectors and the ALiCE® lysate. No additional reagents, no extra steps, and no protein-specific optimizations.
The ALiCE® Kit Workflow
Step 1: Thaw a tube of ALiCE® and add plasmid DNA containing your gene of interest
Step 2: Incubate at 25°C for 24 hours
Step 3: Collect your protein!
Two Pathways of Expression
The ALiCE® kit offers two routes of protein expression for ultimate flexibility:
- For the expression of simple, cytosolic proteins, the pALiCE01 vector is provided. Using this vector, a minimum of 2 mg/ml protein can be achieved.
- For expression of more complex eukaryotic proteins, delivery of your gene in the pALiCE02 vector will target your protein to the microsome (derived from Golgi and endoplasmic reticulum), for post-translational modifications.
Case Studies
Antibodies in a One-pot Reaction
Expression of a functional therapeutic antibody in the ALiCE cell-free protein expression system.
Read the Application Note
GPCR Membrane Proteins
A eukaryotic cell-free platform for reliable expression of functional membrane proteins.
Read the Application Note
Self-assembling Multi-protein Complexes (VLPs)
Expressing Virus-Like Particles using the Cell-Free Protein Expression System.
Read the Application NoteProduce Up to 2 mg/mL of Your Favorite Protein in 24 Hours
The protein produced with the ALiCE® Cell-Free Protein Expression kit can be used for any application, including:
- Structural analysis experiments, including NMR and X-ray crystallography
- Protein: protein interaction studies
- Enzyme assays
- Membrane protein expression and analysis
- Protein activity screening
- Cryogenic electron microscopy
ALiCE® is available as a research kit in two different sizes: midi and maxi to fit your needs.
Got a Question about ALiCE® for Research or Scale-Up?
The Technology behind ALiCE®
ALiCE® is a eukaryotic cell-free protein expression harnessing the power of N. tabacum cells.
Our Technology
ALiCE® as a Protein Service
Leverage our team's expertise for your protein expression project with our end-to-end protein service.
Protein Services