Video: How to Produce Proteins in 24 Hours with ALiCE®
Our ALiCE® cell-free protein expression kits are designed with ease-of-use in mind.
Using our simple protocol will take you from reaction set-up to expressed protein in 24 hours. All it takes is your gene-of-interest cloned into one of our vectors and the ALiCE® lysate. No additional reagents, no extra steps, and no protein-specific optimizations.
The ALiCE® Kit Workflow
Step 1: Thaw a tube of ALiCE® and add plasmid DNA containing your gene of interest
Step 2: Incubate at 25°C for 24 hours
Step 3: Collect your protein!
Two Pathways of Expression
The ALiCE® kit offers two routes of protein expression for ultimate flexibility:
- For the expression of simple, cytosolic proteins, the pALiCE01 vector is provided. Using this vector, a minimum of 2 mg/ml protein can be achieved.
- For expression of more complex eukaryotic proteins, delivery of your gene in the pALiCE02 vector will target your protein to the microsome (derived from Golgi and endoplasmic reticulum), for post-translational modifications.
Case Studies
Antibodies in a One-pot Reaction
Expression of a functional therapeutic antibody in the ALiCE cell-free protein expression system.
GPCR Membrane Proteins
A eukaryotic cell-free platform for reliable expression of functional membrane proteins.
Self-assembling Multi-protein Complexes (VLPs)
Expressing Virus-Like Particles using the Cell-Free Protein Expression System.
Produce Up to 2 mg/mL of Your Favorite Protein in 24 Hours
The protein produced with the ALiCE® Cell-Free Protein Expression kit can be used for any application, including:
- Structural analysis experiments, including NMR and X-ray crystallography
- Protein: protein interaction studies
- Enzyme assays
- Membrane protein expression and analysis
- Protein activity screening
- Cryogenic electron microscopy
Professor Esperanza Rivera de Torre, Assistant Professor, DTU Bioengineering and CTO, AffinityAI
“I was looking for an expression system solution for a high-throughput screening pipeline which had to be GMO. ALiCE® has been a central part of the pipeline development being easy to use in automated systems due to its master-mix ready-to-go formulation and the wide range of proteins that can be expressed efficiently in the system. LenioBio’s team has been extremely helpful in streaming out the protocols and troubleshooting for success.”
Got a Question about ALiCE® for Research or Scale-Up?
The Technology behind ALiCE®
ALiCE® is a eukaryotic cell-free protein expression harnessing the power of N. tabacum cells.
ALiCE® as a Protein Service
Leverage our team's expertise for your protein expression project with our end-to-end protein service.
Product Literature and Case Studies
Access all of our product literature in one place.