Our ALiCE® cell-free protein expression kits are designed with ease-of-use in mind.

Using our simple protocol will take you from reaction set-up to expressed protein in under 48 hours. All it takes is your gene-of-interest cloned into one of our vectors and the ALiCE® lysate. No additional reagents, no extra steps, and no protein-specific optimizations.

Check out our short reaction protocol

The ALiCE® Kit Workflow

  • Step 1

    Thaw a tube of ALiCE® and add plasmid DNA containing your gene of interest

  • Step 2

    Incubate at 25°C for 24 - 48 hours

  • Step 3

    Collect your protein!

Two Pathways of Expression

The ALiCE® kit offers two routes of protein expression for ultimate flexibility:

  1. For the expression of simple, cytosolic proteins, the pALiCE01 vector is provided. Using this vector, a minimum of  2 mg/ml protein can be achieved.
  2. For expression of more complex eukaryotic proteins, delivery of your gene in the pALiCE02 vector will target your protein to the microsome (derived from Golgi and endoplasmic reticulum), for post-translational modifications.

  • A minimum of 2 g/L protein yield​

  • Disulfide bonds & N–glycosylation​

Do you have a question about ALiCE® as a kit? Our experts are happy to answer.

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Think what you could do if you could produce up to 3 g of your favorite protein in under 48 hours…​

The protein produced with the ALiCE Cell-Free Protein Expression kit can be used for any application, including:​

  • Structural analysis experiments, including NMR and X-ray crystallography​
  • Protein: protein interaction studies​
  • Enzyme assays​
  • Membrane protein expression and analysis​
  • Protein activity screening​
  • Cryogenic electron microscopy

ALiCE® is available as a research kit in three different sizes: mini, midi and maxi to fit your needs.

Visit our webshop to explore the options.

Buy ALiCE®

The Technology Behind the Kit

Our cell-free protein expression kits contain our Almost Living Cell-Free Expression (ALiCE®) technology. ALiCE® is a lysate derived from eukaryotic cells and contains all of the protein expression machinery needed to produce even the most difficult-to-express proteins, including those requiring post-translational modifications.

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The technology behind ALiCE®

ALiCE® is a eukaryotic cell-free protein expression harnessing the power of N. tabacum cells.


ALiCE® as a Protein Service

Leverage our team's expertise for your protein expression project with our end-to-end protein service.


Product literature and Case studies

Access all of our product literature in one place.

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