The First Fully Scalable Eukaryotic Cell-Free Expression Platform
From screening campaign to characterization and developability assessment-scale protein production in a matter of days – without the need for cell line development or process optimization. ALiCE®, our pioneering scalable eukaryotic cell-free protein synthesis platform, makes this dream a tangible reality, accelerating protein exploration across a spectrum of applications in basic research to biopharma development.
Challenging the Protein Expression Status Quo
For decades, cell-free protein synthesis has been renowned for its ability to rapidly express proteins resistant to production in cell-based systems. Yet, the challenge of scaling eukaryotic systems, essential for producing complex proteins, has hindered widespread adoption and limited scientists leveraging the full power of this technology. ALiCE® is different: Our team of innovators have developed ALiCE® to work as effectively in microliter volumes as in liter-scale reactions.
One Lysate. Any Scale. Just Add DNA
ALiCE® has been developed for optimal ease of use across scales. From microliter reactions in microtiter plates, through milliliter volumes utilizing conical flaks, to liter-scale reactions in bioreactors, our protocols are optimized for use with standardly available lab equipment. Whatever the scale, it’s the same simple protocol – just add the DNA sequence for your protein and let ALiCE® make your protein.
Contact our team to learn how ALiCE® can work with your established equipment.
Professor Esperanza Rivera de Torre, Assistant Professor, DTU Bioengineering and CTO, AffinityAI
“I was looking for an expression system solution for a high-throughput screening pipeline which had to be GMO. ALiCE® has been a central part of the pipeline development being easy to use in automated systems due to its master-mix ready-to-go formulation and the wide range of proteins that can be expressed efficiently in the system. LenioBio’s team has been extremely helpful in streaming out the protocols and troubleshooting for success.”
Interested in Using ALiCE® for Scaled Protein Production?
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A Transferable Process on Track for GMP
We’ve made significant strides in expanding access to the power of ALiCE®, but our journey is far from over. Our objective is to ensure that ALiCE® is accessible for protein production at any scale, catering not only to research-grade proteins but also to clinical trials and biopharma manufacturing. With a robust ALiCE® manufacturing process in place, we’re forging a clear path towards a fully transferable process and compliance with GMP standards.
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