Protein Expression Kits

Cell-free protein expression kits offering simple reactions with radical results.

Our ALiCE® cell-free protein expression kits are designed with ease-of-use in mind. 


Using our simple protocol will take you from reaction set-up to expressed protein in under 48 hours. All it takes is your gene-of-interest cloned into one of our vectors and the ALiCE®  lysate. No additional reagents, no extra steps, and no protein-specific optimizations. 


Check out our short reaction protocol 


The ALiCE® kit workflow

Describe some quality or feature of the company. Write a short paragraph about it and choose an appropriate icon.

Short title

Step 1

Thaw a tube of ALiCE® and add plasmid DNA containing your gene of interest

Step 2

Incubate at 25°C for 24 - 48 hours

Step 3

Collect your protein!

Two pathways of expression

The ALiCE® kit offers two routes of protein expression for ultimate flexibility:


  1. For the expression of simple, cytosolic proteins, the pALiCE01 vector is provided. Using this vector, up to 3 mg/ml protein can be achieved.
  2. For expression of more complex eukaryotic proteins, delivery of your gene in the pALiCE02 vector will target your protein to the microsome (derived from Golgi and endoplasmic reticulum), for post-translational modifications.


Infographic of the cytosolic and microsomal routes of protein expression in the ALiCE protein expression kit.

Up to 3 g/L protein yield​

Disulfide bonds & N–glycosylation​

Explore our case studies

Antibodies

Antibodies in a one-pot reaction​



Read App note
Membrane Protein

GPCR membrane proteins​

Read App note
Virus-like particles

Self-assembling multi-protein complexes (VLPs) ​

Read App note

Do you have a question about ALiCE® as a kit? Our experts are happy to answer.

Contact ALiCE expert

Think what you could do if you could produce up to 3 g of your favorite protein in under 48 hours...​

The protein produced with the ALiCE  Cell-Free Protein Expression kit can be used for any application, including:​

  • Structural analysis experiments, including NMR and X-ray crystallography​
  • Protein: protein interaction studies​
  • Enzyme assays​
  • Membrane protein expression and analysis​
  • Protein activity screening​
  • Cryogenic electron microscopy

ALiCE® is available as a research kit in three different sizes: mini, midi and maxi to fit your needs.


Visit our web shop to explore the options.


ALiCE cell-free protein expression kit box

The technology behind the kit 


Our cell-free protein expression kits contain our Almost Living Cell-Free Expression (ALiCE®) technology. ALiCE® is a lysate derived from eukaryotic cells and contains all of the protein expression machinery needed to produce even the most difficult-to-express proteins, including those requiring post-translational modifications. 

Cartoon of ALiCE cell-free protein expression kit manufacture
Lab scientists holding ALiCE cell-free protein expression kit

The technology

behind ALiCE®

ALiCE® is a eukaryotic cell-free protein expression harnessing the power of N. tabacum cells. 

Find out how it works
Scientist pipetting into tube from ALiCE cell-free protein expression kit

ALiCE®

as a protein service

Leverage our team's expertise for your protein expression project with our end-to-end protein service. 

Learn more
Contents of the ALiCE cell-free protein expression kit

Product literature

and Case studies

Access all of our product literature in one place. 



Go to resources

Do you have a specific protein that you want to express but you are unsure how?

Contact our experts, and they will help you to identify the best and individual solution for your problem.

LenioBio is committed to protecting your privacy online. Read our Privacy Policy here.

Contact us - ALiCE Kits Page

You can read our privacy policy here.

Share by: