Seamlessly scalable protein production from microliter to liter production
From screening campaign to characterization and developability assessment-scale protein production in a matter of days – without the need for cell line development or process optimization. ALiCE®, our pioneering scalable eukaryotic cell-free protein synthesis platform, makes this dream a tangible reality, accelerating protein exploration across a spectrum of applications in basic research to biopharma development.
Linear scaling of eYFP from microliter to mililiter with ALiCE
Linear scaling of glucose oxidase from microliter to mililiter with ALiCE
Linear scaling of eYFP from microliter to liter scale with ALiCE
For decades, cell-free protein synthesis has been renowned for its ability to rapidly express proteins resistant to production in cell-based systems. Yet, the challenge of scaling eukaryotic systems, essential for producing complex proteins, has hindered widespread adoption and limited scientists leveraging the full power of this technology. ALiCE® is different: Our team of innovators have developed ALiCE® to work as effectively in microliter volumes as in liter-scale reactions.
ALiCE® has been developed for optimal ease of use across scales. From microliter reactions in microtiter plates, through milliliter volumes utilizing conical flaks, to liter-scale reactions in bioreactors, our protocols are optimized for use with standardly available lab equipment. Whatever the scale, it’s the same simple protocol – just add the DNA sequence for your protein and let ALiCE® make your protein.
Speak to our team to learn how ALiCE® can work with your established equipment.
One ALiCE lysate, multiple scales - microtiter plate reactions
One ALiCE lysate, multiple scales - mL in Erlenmeyer flasks
One ALiCE lysate, multiple scales - liter scale bioreactor reactions
We've made significant strides in expanding access to the power of ALiCE®, but our journey is far from over. Our objective is to ensure that ALiCE® is accessible for protein production at any scale, catering not only to research-grade proteins but also to clinical trials and biopharma manufacturing. With a robust ALiCE® manufacturing process in place, we're forging a clear path towards a fully transferable process and compliance with GMP standards.
Want to learn more? Reach out to us.
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