INTRODUCING THE ALiCE® KIT: CELL-FREE EXPRESSION OF COMPLEX PROTEINS AT 3 MG/ML IN BATCH MODE

✓ Up to 30 times more protein at market competitive prices

✓ Active mitochondria drive the reaction

✓ Already validated for over 500 proteins

✓ Post-translational modification (e.g. folding, disulfide bonds, glycosylation, etc)

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of ALiCE® for yourself?

The ALiCE® kit: 30 times more protein than other eukaryotic systems

The ALiCE® kit produces up to 3 mg / mL in batch mode. This is 30 times more than any marketed eukaryotic cell-free system in batch mode (including wheat germ, CHO, human, etc). And, since pricing is similar, you can get up to 30 times more proteins for each euro spent

The ALiCE® kit: Easy to use

ALiCE® is a radically different approach to cell-free expression and a platform unlike any others. Yet, your current cell-free expression processes can be easily translated to ALiCE and you can expect yields you have never witnessed before.

Expression in the cytosolic fraction

  • Simply add the gene (e.g. within a plasmid) to the lysate
  • No need for multiple pipetting steps or other adjustments

Disulfide bonds

  • Simple addition of disulfide bonds
  • Addition of signal peptide triggers directing of proteins to microsomes
  • Mild detergent opens microsomes for collection

ALiCE®: across all industries  and research fields

ALiCE® has been applied for years within Corteva Agriscience, the agrobioi division of DowDoPont, as well as Fraunhofer IME to express over 500 proteins of varying types. This shows that ALiCE® is applicable for any purpose, be it for biopharmaceuticals, technical enzymes, crop development, metabolic pathways, fundamental research, etc.

Examples – A closer look on proteins expressed in ALiCE®

Full Size Antibody M12

  • 0.14 mg/mL protein in an earlier version of ALiCE®*
  • Hetero Tetramer which contains disulfide bonds
  • Directed to microsome for folding
  • Functionality verified with ELISA testing *Buntru et al., Biotechnology and Bioengineering, 2015, Vol. 112, No. 5

Glucose oxidase

  • 1mg/mL protein
  • Homodimer which contains disulfide bonds
  • Directed to microsome for folding
  • Activity determined with colorimetric assay

Enhanced Yellow Fluorescent Protein (eYFP)

  • 3mg/mL protein
  • Has been expressed in 3 mL reactions
  • Monomer which contains no disulfide bonds
  • Activity determined with fluorescent assay

Membrane protein HB-EGF fused to eYFP

  • Heparin Binding – Epidermal Growth Factor – eYFP
  • Full size protein expressed with only minor signs of
  • fragmentation
  • Protein incorporated into the microsomal membrane (arrow)

Ready to try ALiCE® for yourself?
Order now or contact an expert with your questions.